![]() ![]() Bi-monthly case conference at the board meeting. Simhaee looks forward to meeting you and developing a connection that will. Outstanding Course Director, Course of the Year Award (awarded to Top 2 of 200 CME courses) Mayo School of Continuous. Top 40 Under 40, Ranked 7 The Ophthalmologist. Top Doctors, Metro Area Ophthalmology Castle Connolly Medical Ltd. This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017. Founded in 2002, Midwest Retina (MWR) has grown quickly into the largest retina-only private practice in. NYU Grossman School of Medicine Residency, Ophthalmology, 2014 2017. Top Doctor, Ophthalmology Castle Connolly Medical Ltd. Future study will evaluate the quality of protein detection with ELISA assays after using these methods Vitreous sample sonication or lysis buffer extraction significantly improves the consistency of protein measurement of samples. These were not statistically significant compared to control (p=0.3988 and p=0.2464, respectively). Charter Inductee of the Retina Hall of Fame 2017 OSN Top 150 of Retina Specialist 2016 American Academy of Ophthalmology Senior Achievement Award, 2011. Garretson is a member of the Alpha Omega Alpha Medical Honor Society. The mean CV of vortex treatment and hand-held homogenization were 17.05% ± 7.73 and 18.43% ± 3.28, respectively. He has been named to The Best Doctors in the Midwest, and The Best Doctors in America. The mean CV of lysis buffer treatment and sonication are significantly lower than that of control (4.34% ± 1.79, P=0.0417 and 4.49% ± 3.14, p=0.0449, respectively). The mean CV for the no treatment control was 13.48% ± 9.21. Student’s t-test was used for statistical analysis. The coefficient of variation (CV %) from the three assays was averaged between the 4 subjects and compared between the 5 methods. Samples in each assay were run in triplicate accounting for pipetting error. Three independent assays were run with each treatment and all 4 subjects. BCA assay with 96 well plates was performed for the comparison of five groups of vitreous preparation: 1) vortex (S3200, BioExpress), 2) hand held-homogenization (Disposable pellet mixer and cordless motor, VWR), 3) sonication (Dismembrator-500, Fisher Scientific), 4) the use of lysis buffer (9803S, Cell signaling), and 5) no treatment as a control. ![]() Patient vitreous samples (n=4 /group) were randomly selected from sample collection under an IRB-approved protocol during retinal detachment surgical procedure. The purpose of this study is to compare multiple methods of vitreous sample preparation for the consistency of measured protein levels between multiple assays. Romano, MD, PhD CRISPR/Cas9 Shows Promise for Patients with LCA Mark E. Vitreous structure can hinder the measurement of consistent protein concentration levels over subsequent assays for research or clinical testing in retinal detachment. EURETINA Congress - Eyetube Meeting Coverage: EURETINA Congress EURETINA: 2022 The Coming Shift in AMD Care Anat Loewenstein, MD, MHA and Alistair Laidlaw, MBBS, MD Treating Traumatic Aphakia and Aniridia Matteo Forlini, MD and Mario R. ![]()
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